What is
Palo Negro

Palo Negro is the common name of the Chilean shrub Leptocarpha rivularis, which contains Leptocarpina naturally. This shrub grows between Bio Bio and Osorno (VIII and X regions of Chile), in mountain range, next to water courses and, generally, to semi shade.


It is a bush of black branches, opposite leaves with little dentures and bright yellow flowers. Its consumption is indicated as a preventive and adjuvant in the treatments of Cancer and Diabetes. It is also a good liver cleaner, anti-inflammatory and controls cholesterol. Agrícola Los Esteros is the only producer in the world of Palo Negro.

What is

It is a molecule with low toxicity, high specificity and efficiency. Scientific studies demonstrate through extensive research developed in four different Chilean universities, that Leptocarpina is a molecule that contributes positively and strongly in the prevention and treatment of Cancer and Diabetes, in a wide range of cancer lines studied in vitro and in vivo.


They conclude that Leptocarpine possesses cytotoxic, apoptotic and chemosensitizing activity on leukemic and tumor cells of different strains, which supports its use as a preventive and adjuvant. In turn, the studies show strong results regarding their hypoglycemic function, without any short or medium term side effects, which makes Palo Negro a powerful and cutting-edge product in the fight against and prevention of Type Diabetes 2.

What does it mean that Leptocarpine has cytotoxic and apoptotic activity?

The action of Leptocarpine is to cause a stress in the diseased cell, which immediately and thanks to the action of the molecule activates the genes responsible for its repair and those in charge of regulating the DNA cell cycle.

When the cellular repair fails, thanks to the action of the molecule Leptocarpina, the genes responsible for causing the death of the diseased cells (apoptotic) act, also proceeding the genes whose mission is to keep the healthy cells (Proapoptóicos and antiapoptóicos). This explains the most revolutionary of the Palo negro: it destroys diseased cells without affecting the healthy cells that interact with Leptocarpina.

How does it

According to the studies Leptocarpina’s action consists in causing a stress (pressure) on the diseased cell that immediately activates the genes responsible for its repair and regulate the DNA cell cycle.

The professor of the Department of Chemistry of the Universidad Andrés Bello explains that when there is no possibility of repair proapoptotic genes (the genes responsible for cell death) and antiapoptotic genes (whose mission is to keep the cell alive) come into play. In cases where the proapoptotic gene predominates, the cell dies. However, what is significant is that healthy cells are not affected by this system.

Professor Martínez puts it this way: “The exposure of cells to genotoxic stress causes activation of the protein kinase pathway (SAPK) and induction of DNA repair enzymes and cell cycle regulatory proteins, such as p53 and p21waf1. The tumor suppressor protein p53 transmits signals that activate p21waf1 gene expression. The p21waf1 protein then limits the progression of the cell cycle, thus leaving time for DNA repair to occur.


Rolando Martinez. Andrés Bello University, Santiago, Chile.

Mapuche ancestral medicine and university

biotechnological research after cure for cancer.

  • Mena, C. (2008). Efecto apotótico y quimiosensibilizador de Leptocarpina en distintos modelos celulares de leucemias. UACH

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  • Contreras, C. (2005). Efecto hipoglucemiante de la infusión de Leptocarpha rivularis en ratas sprague-dawley diabéticas Tipo II por induccion con aloxano. UACH

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  • Ríos, A. (2008). Síntesis, caracterización y ensayos biológicos de isoxazoles con células cancerígenas de leucemia promielocítica humana, hl-60.UACH

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  • Martínez, R. Kesternich, V. Carrasco, H. Álvarez, C. et al (2006). Synthesis and conformational analysis of Leptocarpin. Derivatives. Influence of modification of the oxirane ringon Leptocarpin’s cytotoxic activity. Chile: Universidad Austral, Universidad Católica del Norte, Universidad Andrés Bello.

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